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 Maine Department of Environmental Protection
 Biological Monitoring Program
 Stream Algae Report

Site Information
Name: Big Brook - Station 728 Sampled by: Biomonitoring Unit
Town: Madawaska Method: Rock Scrapings 1 Inch Circles
Biomonitoring Basin St. John And Allagash Sample ID: SA-728-2014R (1388)
Sample Date: 2014-07-09 Taxonomist: Academy Of Natural Sciences
Location: 22m Downstream Of Lavert Road
Sample comments:
Latitude: 47.25914243
Longitude: -68.25961263

Three figures from left to right: On the far left, an outline of the state of Maine with the site location marked in blue. In the middle, a satellite image view of the area of the site, with waterbodies marked in blue, and the site marked in red, with a scale bar and north arrow. On the right, a downstream image of the sample site.

Model Results and Variable Plots

 Blue = this sample. Boxplots = range of all samples. Grey shaded areas = 90th and/or 10th percentile of reference site values.

Statutory Class = B
Final Determination = C
Attains Class = NO
Reason = Model
Model Result = C
Prob. Class A = 0.00
Prob. Class B = 0.03
Prob. Class C = 0.96
Prob. NA = 0.01

Boxplots of stream algae model metrics, including range of values from all samples, from reference sites, and this specific sample. Stream algae model metrics are in a table below.

Additional Variable Plots

 Blue = this sample. Boxplots = range of all samples. Grey shaded areas = 90th and/or 10th percentile of reference site values.

Boxplots of additional stream algae metrics, including range of values from all samples, from reference sites, and this specific sample. Additional stream algae metrics are in a table below.

Model Variables Table
Variable Sample value All data median All data 10th percentile All data 90th percentile Reference range lower limit Reference range upper limit Reference range description
Salt-tolerant diatom percent abundance 1.01 0.90 0.00 9.15 0.00 1.08 <90th percentile
Nutrient-tolerant diatom richness 11.00 8.00 3.00 17.00 0.00 8.00 <90th percentile
Intermediate diatom richness 20.00 19.00 11.00 28.80 9.00 25.30 10th to 90th percentile
Intolerant algae percent species 6.06 18.75 3.70 42.27 22.12 72.41 >10th percentile
Motile diatom percent abundance 30.02 8.49 1.55 31.56 0.00 11.59 <90th percentile
Tolerant algae percent abundance 5.28 1.19 0.00 17.43 0.00 1.05 <90th percentile
Additional Variables Table
Variable Sample value All data median All data 10th percentile All data 90th percentile Reference range lower limit Reference range upper limit Reference range description
Tolerance Index (all algae) 44.95 42.67 37.09 49.07 0.00 42.34 <90th percentile
Nitrogen Index (all algae) 47.76 38.64 26.81 51.56 0.00 35.58 <90th percentile
Phosphorus Index (all algae) 69.40 58.62 38.59 76.56 0.00 53.06 <90th percentile
Intolerant algae percent species 2.00 6.00 1.00 14.00 5.00 23.00 >10th percentile
Intolerant algae richness 9.68 6.25 0.00 16.00 0.00 7.14 <90th percentile
Salt-tolerant diatom percent richness 94.61 89.95 61.14 98.30 45.42 97.96 10th to 90th percentile
Tolerant algae percent richness 33.33 16.67 0.00 41.38 0.00 14.29 <90th percentile
Nutrient- tolerant diatom percent abundance 20.51 42.06 7.59 84.31 0.00 48.33 <90th percentile
Stream Characteristics and Water Chemistry Plots

 Blue = this sample. Boxplots = range of all samples. Grey shaded areas = 10th to 90th percentile of reference site values

Bankfull width = 6 m
Wetted width = 6 m
Depth = 30 cm
Water velocity = 65 cm/s
Riffle = 100 %
Canopy Cover = 29 %
Boulder = 50 %
Cobble = 30 %
Gravel = 15 %
Sand = 5 %
Boxplots of stream water chemistry. Values are in a table below.
Water Chemistry Table
Parameter Sample Date Sample value All data median All data 10th percentile All data 90th percentile Reference 10th percentile Reference 90th percentile Units
Dissolved Oxygen 2014-07-09 8.1 8.60 7.00 10.23 7.80 10.62 Milligrams Per Liter
pH 2014-07-09 7.04 7.12 6.21 7.97 6.00 7.55 Standard Ph Unit
Specific Conductance 2014-07-09 109 88.55 25.00 492.00 15.41 67.31 Microsiemens Per Centimeter
Temperature 2014-07-09 21.3 20.00 14.70 24.70 12.80 23.60 Degrees Celcius (Temperature)
Nitrate + Nitrite As Nitrogen 2014-07-09 0.45 0.08 0.02 0.61 0.01 0.09 Milligrams Per Liter
Orthophosphate As Phosphorus 2014-07-09 4 2.00 1.00 11.00 1.00 3.00 Micrograms Per Liter
Total Alkalinity 2014-07-09 44 19.50 4.44 113.20 2.00 21.00 Milligrams Per Liter
Total Kjeldahl Nitrogen 2014-07-09 0.5 0.40 0.20 0.60 0.10 0.50 Milligrams Per Liter
Total Phosphorus 2014-07-09 25 18.00 7.00 48.00 4.00 18.00 Micrograms Per Liter
Viewing Bucket
Viewing Bucket Table
Cover type Percent cover Cover group
Filament 5-15cm Long 0.95 Nuisance algae
Moss 6.67 Not algae
Sand / Mud 4.13 Not algae
Mat Incomplete 77.78 Thin algal layer
Mat <1mm Thick 10.48 Thin algal layer

Barplot of viewing bucket substrate cover types, available in table on the right.

Land Use
Land Cover Data Source: MELCD 2004
Watershed Area (sq km): 30.7
Impervious Cover %: 1
Land Use Table
Cover Type Percent Category
Highly Urban 0.03 Developed
Urban 0.20 Developed
Residential 1.38 Developed
Water 0.84 Natural
Wetland 3.99 Natural
Forest 58.48 Natural
Grass 8.49 Agriculture
Cleared 0.02 Agriculture
Tilled 26.54 Agriculture

Barplots of landcover data from watershed upstream of sample location. Percentages are available in a table on the right

Site Information
Name: Big Brook - Station 728 Sampled by: Biomonitoring Unit
Town: Madawaska Method: Rock Scrapings 1 Inch Circles
Biomonitoring Basin St. John And Allagash Sample ID: SA-728-2014R (1388)
Sample Date: 2014-07-09 Taxonomist: Academy Of Natural Sciences
Taxa List (See key at end of report)

 Tolerance values color coding: Blue for sensitive, <32.20. Purple for intermediate, >=32.20 and <60. Red for tolerant, >=60.

Name Group RA RB Oxy Sap Sal Tr Mot GF Tol TN TP
Phormidium Filamentous Cyanobacteria 0.420 0.091 42.6 60
Unknown Rhodophyte Florideophycidae (Chantransia) Red Algae 0.256 0.313 47.5 48 68
Pseudanabaena Filamentous Cyanobacteria 0.101 0.003 NA 71 84
Achnanthidium minutissimum Pennate Diatom 0.079 0.006 1 2 2 7 N P 41.2
Phormidium formosum Filamentous Cyanobacteria 0.064 0.004 42.6 23
Reimeria sinuata Pennate Diatom 0.035 0.014 1 2 2 3 M P 60.9 65 88
Encyonema silesiacum Pennate Diatom 0.006 0.005 3 3 2 7 V S 46.9 43 69
Fragilaria capucina Pennate Diatom 0.004 0.002 2 2 3 N V 32.9 59 51
Planothidium frequentissimum Pennate Diatom 0.003 0.001 3 4 2 V P 65.1 69 92
Gomphonema parvulum Pennate Diatom 0.003 0.002 4 4 2 5 N S 42.2 21
Navicula cryptotenella Pennate Diatom 0.003 0.002 2 2 7 M P 44.6 61 82
Nitzschia dissipata Pennate Diatom 0.003 0.002 2 2 2 4 H P 60.9 48 75
Staurosira construens var. venter Pennate Diatom 0.002 0.000 1 2 2 4 N V 41.3 35 67
Closterium lunula Desmid 0.002 0.520 NA
Cocconeis lineata Pennate Diatom 0.002 0.007 3 2 2 5 N P 51.5 62 82
Encyonema minutum Pennate Diatom 0.002 0.001 2 V S 50.2 21
Fragilaria vaucheriae Pennate Diatom 0.002 0.001 3 3 2 5 N V 44.2 36
Nitzschia palea Pennate Diatom 0.002 0.001 4 5 2 6 H P 60.3 67 89
Psammothidium subatomoides Pennate Diatom 0.001 0.000 1 1 1 2 V P 42.4 20 35
Achnanthidium deflexum Pennate Diatom 0.001 0.000 1 2 3 N P 43.5 40 52
Achnanthidium rivulare Pennate Diatom 0.001 0.000 N P 49.9 39 49
Encyonopsis subminuta Pennate Diatom 0.001 0.000 V S NA 30 36
Eolimna minima Pennate Diatom 0.001 0.000 4 4 2 5 M P 57 64 88
Fragilaria crotonensis Pennate Diatom 0.001 0.001 2 2 2 3 N V NA 53 64
Gomphonema kobayasii Pennate Diatom 0.001 0.000 N S 71.2 50 79
Melosira varians Centric Diatom 0.001 0.013 3 3 2 5 N U 67 67 89
Navicula cryptocephala Pennate Diatom 0.001 0.001 3 3 2 7 M P 56.3 55 80
Navicula lanceolata Pennate Diatom 0.001 0.004 3 3 3 5 M P 77.5 75 98
Eucocconeis laevis Pennate Diatom 0.001 0.000 1 1 1 1 V P 29.5 17 18
Tabellaria flocculosa Pennate Diatom 0.000 0.000 1 2 1 3 N U 18.8 17 32
Ulnaria ulna Pennate Diatom 0.000 0.000 3 4 2 N E 34.2 17 21
Adlafia minuscula Pennate Diatom 0.000 0.000 4 2 1 1 M P NA 96
Amphipleura pellucida Pennate Diatom 0.000 0.000 2 4 2 2 M P 43.8 20 59
Cocconeis placentula Pennate Diatom 0.000 0.000 3 2 2 5 N P NA 30 82
Cyclotella meneghiniana Centric Diatom 0.000 0.001 5 4 3 5 N U 72.6 55 73
Gomphonema olivaceoides Pennate Diatom 0.000 0.000 1 1 1 3 N S 70.8 63 55
Navicula trivialis Pennate Diatom 0.000 0.001 3 3 3 5 M P 78.6 68 98
Navicula viridula Pennate Diatom 0.000 0.001 2 3 2 5 M P 70.7 74
Nitzschia linearis Pennate Diatom 0.000 0.002 2 2 2 4 H P 54.2 68 87

Taxa List Key
Column Name Description
RA Relative Abundance number of cells (density of cells per square centimeter) in a sample / total abundance of all taxa
RB Relative Biovolume Volume of a taxon’s cells in a sample / volume of all algae cells in a sample
Oxy van Dam Oxygen tolerance 1=100% DO saturation, 2=>75% DO saturation, 3=>50% DO saturation, 4=>30% DO saturation, 5=<30% DO saturation
Sap van Dam Saprobic value Saprobic values relate to the amount of organic pollution, biological oxygen demand, and DO concentrations. Values range from 1 (intolerant of organic pollution) to 5 (highly tolerant of organic pollution)
Sal van Dam Salinity value 1=freshwater (<100 mg/L chloride) , 2=fresh-brackish (<500 mg/L), 3=brackish-freshwater (500-1000 mg/L), 4=brackish (1000-5000 mg/L)
Tr van Dam Trophic value 1=oligotraphentic, 2=oligo-mesotraphentic, 3=mesotraphentic, 4=meso-eutraphentic, 5=eutraphentic, 6=hypereutraphentic, 7=indifferent to trophic state
Mot Motility of Diatoms Motility (capability of movement in water or on substrates) based on genus (Wang et al. 2005): N= not motile, M = motile , H = highly motile, V=variable within genus
GF Growth Forms E= erect, P=prostrate, S=stalked, U=unattached, V=variable (based on communication with R. Jan Stevenson)
Tol Tolerance value Tolerance to a combination of watershed disturbance, conductivity, and nutrient enrichment (based on Maine data). Value range from 1=most senstive to 100=most tolerant.
TN TN value Preference for total nitrogen (TN) based on Maine data. Values range from 1=low TN concentrations to 100=high TN concentrations.
TP TP value Preference for total phosphorus (TP) based on Maine data. Values range from 1=low TP concentrations to 100=high TP concentrations.